This report covers work done in a collaborative study to compare results from cigarette-machine puffing of e-cigarettes under a range of puffing parameters to make a recommendation for standardised parameters. The study results demonstrated that any of the regimes evaluated gave similar results but that one would be more suitable for machine-testing of e-cigarettes of a conventional design for the determination of major constituents in trapped aerosol. Thus the favoured parameters (55mL/3s/30s/square) will serve as a recommended machine regime for e-cigarette aerosol collection.