2016 Collaborative Study of Humectants - Determination of Glycerol and 1,2-Propylene Glycol in Tobacco
RAC Technical Report
In 2012, the CORESTA Smokeless Tobacco Sub-Group (now the Tobacco and Tobacco Product Analytes Sub-Group [TTPA]) conducted a collaborative study to evaluate the suitability of the CORESTA Recommended Methods (CRMs) Nos. 60 and 61 for the determination of two humectants, 1,2-propylene glycol (propylene glycol or PG) and glycerol (Gly), in smokeless tobacco products.
In 2015, the Routine Analytical Chemistry Sub-Group (RAC) proposed amending CRM 60 with the goal to increase the extraction efficiency of the GC method by adding water to samples prior to extraction with methanol. In 2016 a collaborative study was conducted to allow the repeatability and reproducibility to be calculated for the proposed revised method and compare the results to those obtained using CRM 61.
This report summarises the findings of the 2016 collaborative study, which shows that the repeatability (r) and reproducibility (R) data obtained for the proposed revised CRM 60 are comparable to that obtained previously for both the GC (CRM 60) and LC (CRM 61) methods undertaken in previous studies. CRM 60 will be updated, but due to the low number of participants that used the LC technique for this study an update of CRM 61 is not planned.
CRM 61 - Determination of 1,2-Propylene Glycol, Glycerol and Sorbitol in Tobacco and Tobacco Products by High Performance Liquid Chromatography (HPLC) (July 2015)
CRM 60 - Determination of 1,2-Propylene Glycol and Glycerol in Tobacco and Tobacco Products by Gas Chromatography (July 2015)
Report - 2012 Collaborative Study on Humectants (February 2015)
Report - Technical Report on 2007 Joint Experiment to Update Repeatability and Reproducibility Statistics for CRM 60 and CRM 61 (January 2010)