From 2013 to 2016, the CORESTA Routine Analytical Sub-Group (RAC) and Smokeless Tobacco Sub-Group (STS) conducted a series of collaborative studies for the determination of nicotine in tobacco and tobacco products including cigarette and cigar filler, and smokeless tobacco products. A gas chromatography mass spectrometry (GC-MS) method using methanol as the extract solvent and quinoline as the internal standard was eventually selected.

The purpose of this study was to evaluate repeatability and reproducibility (r & R) values of the methodology and draft a new CORESTA Recommended Method (CRM) for the determination of nicotine in tobacco and tobacco products.