In October 2018, the CORESTA Tobacco and Tobacco Products Analytes Sub-Group (TTPA) initiated a collaborative study for the determination of nicotine in traditional and very low nicotine (VLN) tobacco and tobacco products. The intent of this study was to lower the calibration range for two CORESTA Recommended Methods, CRM 62 and CRM 87, for the analysis of VLN tobaccos and tobacco products. This project was important to ensure these two CRMs are relevant for emerging regulations to reduce nicotine for combusted tobacco products. The results of the study demonstrate that, with modifications, CRM 62 and CRM 87 are suitable and the TTPA SG recommended that the calibration ranges of the two CRMs be lowered to include VLN tobacco and tobacco products.