2019 Small Group Collaborative Study on Aromatic Amines in Mainstream Cigarette Smoke
SMA Technical Report
From 2014 to 2017, the CORESTA Special Analytes Sub-Group (SPA) (now named Smoke Analytes Sub-Group, SMA) evaluated analytical methods for quantitative measurement of aromatic amines in mainstream cigarette smoke.
At the beginning of the project, a survey was conducted to get an insight into methodologies used for measurement of aromatic amines by the SG. Most laboratories employed methods using Gas Chromatography Mass Spectrometry (GC/MS), but a group of laboratories used methods based on Liquid Chromatography Tandem Mass Spectrometry (LC‑MS/MS). The GC/MS method was taken forward for a Collaborative Study (CS) and the results published in a Technical Report. The learnings from LC-MS/MS based methods were also summarised in a separate Technical Report.
The GC/MS results obtained through the CS did not support development of the method into a CRM due to large variation and high repeatability (r) and reproducibility (R) values. However, the SG agreed that the analytical method would benefit from further investigative work to improve / decrease r&R values, if possible.
A focus group volunteered to continue working on the method investigation and results concluded that modifications did not result in significant improvements to r&R that would warrant another full collaborative study. This report documents the method as written for the original 2016 CS and captures the work of the focus group.
CRM 95 - Determination of Aromatic Amines in Mainstream Cigarette Smoke by Gas Chromatography Mass Spectrometry with Negative Chemical Ionisation (GC/MS(NCI)) (January 2021)
Report - 2016 Joint Experiment on Aromatic Amines in Mainstream Cigarette Smoke by LC-MS/MS (November 2019)
Report - 2016 Collaborative Study on Aromatic Amines in Mainstream Cigarette Smoke (March 2019)
Report - Determination of Aromatic Amines in Cigarette Mainstream Smoke. The CORESTA 2007 Joint Experiment (July 2010)