2019 Small Group Collaborative Study on Aromatic Amines in Mainstream Cigarette Smoke
SMA Technical Report
From 2014 to 2017, the CORESTA Special Analytes Sub-Group (SPA) (now named Smoke Analytes Sub-Group, SMA) evaluated analytical methods for quantitative measurement of aromatic amines in mainstream cigarette smoke.
At the beginning of the project, a survey was conducted to get an insight into methodologies used for measurement of aromatic amines by the SG. Most laboratories employed methods using Gas Chromatography Mass Spectrometry (GC/MS), but a group of laboratories used methods based on Liquid Chromatography Tandem Mass Spectrometry (LC‑MS/MS). The GC/MS method was taken forward for a Collaborative Study (CS) and the results published in a Technical Report. The learnings from LC-MS/MS based methods were also summarised in a separate Technical Report.
The GC/MS results obtained through the CS did not support development of the method into the CRM due to large variation and high repeatability (r) and reproducibility (R) values. However, the SG agreed that the analytical method would benefit from further investigative work to improve / decrease r&R values, if possible.
A focus group volunteered to continue working on the method investigation and results concluded that modifications did not result in significant improvements to r&R that would warrant another full collaborative study. This report documents the method as written for the original 2016 CS and captures the work of the focus group.
Report - 2016 Joint Experiment on Aromatic Amines in Mainstream Cigarette Smoke by LC-MS/MS (November 2019)
Report - 2016 Collaborative Study on Aromatic Amines in Mainstream Cigarette Smoke (March 2019)
Report - Determination of Aromatic Amines in Cigarette Mainstream Smoke. The CORESTA 2007 Joint Experiment (July 2010)