The requirements for mainstream emissions testing of products other than conventional cigarettes, continues to increase. Methods initially developed for the determination of mainstream cigarette emissions provide a good foundation for the analysis of next generation nicotine products including heat-not-burn (HnB) and e-vapour products, as well as other types of burning products (e.g. cannabis). Adaptations to extend the scope of these methods to other mainstream emissions, require demonstrating the performance characteristics of the method are unaffected. To demonstrate a change in the matrix did not impact extraction efficiency or the instrumental analysis of the analytes, either an artificial matrix or test sample matrix was used. The determination of lab fortified matrix recoveries, as well as the determination of resolution and tailing factor for chromatographic methods, were compared against conventional products. Trapping efficiency experiments were also performed to demonstrate the collection system was adequate for the collection of particulate phase and vapor phase analytes in these emissions. For the assessment of the analytical performance, multiple approaches were required depending on the product type, levels of the analyte of interest, and potential contributions from sources other than the test samples. Combinations of assessing laboratory reagent blanks, lab fortified blanks, lab fortified matrices and air blanks were required, depending on the specific scenario. Excluding any modifications or adaptations to enhance limits of quantification, methods developed for the analysis of mainstream emissions from cigarettes, were directly applicable to next generation nicotine products, as well as other types of burning products.