Chalara elegans is a cool climate parasite of many cultivated and wild growing plants world-wide including tobacco. The fungus causes necrotic lesions on roots that reduce the yield and quality of leaves. One of interesting sources of black root rot resistant germplasm is wild relative of tobacco Nicotiana glauca . Interspecific hybrids of N. tabacum cv. Wislica x N. glauca (WGL) were previously produced to transfer N. glauca resistance genes into tobacco. In this study ten breeding lines of BC₂F₅ - BC₂F₇ generation carrying black root rot resistance were evaluated for growth, development and chemical characteristics. Plant height and number of leaves per plant in all WGL lines were similar to that of parental cultivar Wislica. There were some significant variations for leaf characteristics. The most distinct effect was extended duration of the vegetative stage. All the WGL lines showed delayed flowering compared to Wislica. The WGL lines were inferior to the parental cultivar with regard to weight of 10 dm₂ of leaf blade. The cured leaf yield of most of the WGL lines was higher than that of Wislica. There were no major differences for nitrogen, sugars and nicotine contents. The results from the study indicate that incorporation of the N. glauca -type resistance into tobacco genome did not cause unambiguously compromising effects on the usability of tobacco.