TSRC, Tob. Sci. Res. Conf., 2022, 75, abstr. 77

Analysis of aromatic aldehydes in e-vapor products by GC-MS/MS

SIRKISOON L.; VIA L.; ZHU J.; TAYYARAH R.
ITG Brands, Greensboro, NC, USA

Aromatic aldehydes (furfural, benzaldehyde, cinnamaldehyde, ethyl vanillin, and vanillin) are of interest for e-vapor products testing based on recent guidance from FDA‐CTP. There are limited reports of direct application methods that include each of these analytes of interest in one method. Here we present a simple GC‐MS/MS method for the analysis of these aromatic aldehydes in e‐vapor product aerosols and e‐liquids with relatively low analyte LOQs. The method was developed using acetone as the solvent and 2,6‐Di‐tert‐butyl‐4‐methylphenol (BHT) as an additive to prolong the stability of ethyl vanillin and vanillin. Split injection mode (50:1) is utilized and the thermostability of these compounds allowed for a high inlet temperature (325°C), which is necessary for rapid vaporization of the analytes and removal of propylene glycol and glycerol from the inlet. Additionally, optimization of the source temperature revealed that 350°C is most suitable for the five analytes of interest. The calibration range for furfural, benzaldehyde, and cinnamaldehyde is 8 to 8000 ng/mL, and 100 to 100000 ng/mL for ethyl vanillin and vanillin. Two internal standard compounds are used; Furfural‐d4 for furfural, benzaldehyde and cinnamaldehyde, and vanillin‐d3 for ethyl vanillin and vanillin. The LOQ of the method is defined as the lowest spiked analyte concentration during the validation which was 8.08 ng/mL, 0.003232 μg/puff for 50 puffs of aerosols, or 0.4489 μg/g of e‐liquid for furfural, benzaldehyde, and cinnamaldehyde, and 101 ng/mL, 0.0404 g/puff for 50 puffs of aerosols, or 5.6111 μg/g of e‐liquid for ethyl vanillin and vanillin. This new method was validated with good recoveries for e‐vapor product aerosols (97‐127%) and e‐liquids (89 ‐ 128%).