TSRC, Tob. Sci. Res. Conf., 2009, 63, abstr. 08

Analysis of pesticides in smokeless tobacco extracts by comprehensive multi-dimensional gas chromatography-time of flight mass spectroscopy (GCxGC-TOFMS)

BINKLEY J.; PUGH S.
LECO Corporation, Saint Joseph, MI, USA

Smokeless tobacco is known to contain several thousand analytes making it an extremely difficult matrix in which to identify pesticide residues. Current methodologies incorporate the use of complex, time consuming sample cleanup techniques to eliminate much of the matrix interference prior to GC-MS analysis. This poster will show how the increased peak capacity of comprehensive multi-dimensional gas chromatography provides the ability to effectively separate pesticides from complex sample matrix while minimizing the need for extensive sample cleanup methods. In addition, the cryo-focussing effects of thermal modulation increases detectability allowing lower levels of these pesticide residues to be determined. The use of a Time of Flight Mass Spectrometer provides the ability to acquire full mass range spectra without sacrificing sensitivity. This is beneficial for detecting not only target pesticide analytes, but also new and emerging contaminants.The GCxGC-TOFMS data from two commercially available smokeless tobacco products will be discussed. Various QuEChERS sample preparation techniques were used for this work and will be shown in this poster.