Analysis of saccharide in tobacco by High Performance Liquid Chromatography with Evaporative Light Scattering Detection
A high performance liquid chromatographic method (HPLC) with evaporative light scattering detection (ELSD) for the determination of saccharide in tobacco was developed. Two of monosaccharide: glucose, fructose and two of disaccharide: sucrose and maltose in tobacco were analyzed. The tobacco products after drying were smashed and filtered by 40 meshes sieve. The sample powder was extracted by 80% ethanol under the condition of high pressure (1500 PSI) and comparatively high temperature (80 °C) through Accelerated Solvent Extractor, and then the protein in extracted solvent was precipitated by PbAc2 and filtered out. The final solvent after volume adjustment was analyzed through HPLC with evaporative light scattering detection. The experiment data indicated that the method had the detection limit of less than 0.003 mg/mL, the recovery fell between 93% and 104%, and the RSD (n=8) less than 5% in which glucose, fructose and sucrose less than 3%. Applying the method for determining above 4 saccharide in 15 cigarette products and 46 different type of tobacco samples were determined. The results showed that the applying ELSD in saccharide determination of tobacco products had the advantage of stability, rapidness and exactness.