Analysis of the TPD2-related flavor compounds in cigarettes by GC-MS/MS and LC-MS/MS
The new European Union Tobacco Products Directive (TPD2) came into force in May 2016. The European Commission has established a priority list of 15 additives referred to in Article 6 of TPD2. The purpose of this study was to develop quantitative analysis methods of three flavor compounds (geraniol, maltol and guaiacol) among the additives in the priority list because there is no international standard method for their quantification for cigarettes. In order to obtain highly selective analytical results based on their chemical properties, the gas chromatography tandem mass spectrometry (GC-MS/MS) method was used for guaiacol and geraniol, and the liquid chromatography tandem mass spectrometry (LC-MS/MS) method was used for maltol. For quantitative analysis, methanol extracts and water-acetonitrile (9:1, v/v) extracts from cigarette samples were used for GC-MS/MS and LC-MS/MS, respectively. The results showed that guaiacol and geraniol had good correlation coefficients (R2 > 0.999) and good average recoveries (addition concentration of 0.5 µg/g) of 99.8 % and 101 % with relative standard deviations (RSD) of 3.8 % and 0.8 %, respectively. Commercial and reference cigarettes (CM8 and 3R4F) contained 1.32 µg/g to 1.45 µg/g and 0.06 µg/g to 0.17 µg/g of guaiacol and geraniol, respectively. The results also showed that maltol had a good correlation coefficient (R2 > 0.999) and good average recoveries (addition concentration of 0.5 µg/g) of 101 % with RSD of 5.3 %. Commercial and reference cigarettes contained 1.22 µg/g to 3.62 µg/g of maltol. These methods showed high selectivity, good linearity, and high reproducibility for analysis of geraniol, maltol, and guaiacol.