Application and use of e-cigarettes HPHC methods for the analysis of heated tobacco products
The interest in e-cigarette research has resulted in the development of methods for the quantitative analysis of chemical constituents present in their aerosols. The emissions of e-cigarettes contain not only nicotine, propylene glycol, glycerin, water and flavours, but also nicotine related impurities and thermal degradation products. We have developed and validated analytical methods for the analysis of e-cigarette aerosols including the compounds listed in the U.S. FDA Draft PMTA guidance document. These include primary constitutes, aldehydes, VOCs, organic acids, metals, aromatic amines, nicotine related impurities and benzo[a]pyrene. An emerging tobacco product; heated tobacco products (HTPs) produce an aerosol by the heating of tobacco. Since tobacco is not combusted in these products, the majority of the resulting aerosol is comprised of water, humectants and volatile compounds. Given the similarities between e-cigarettes and HTPs, we hypothesized that e-cigarettes methods would also be suitable for analysis of HTPs emissions. In this study, we have applied our e-cigarette methods to the analysis of the iQOS HEETS HTPs. The aerosols were collected under intense smoking condition, using a device holder designed for e-cigarettes, and analyzed for HPHCs. We found that methods developed and validated for e-cigarettes could be successfully applied for the analysis of HTPs emissions. With the exception of nicotine and water, the emissions from HTPs were found to be more similar in emissions to e-cigarette aerosol than cigarette smoke. We found that e-cigarette methods were well suited for the analysis of HTPs due to the lower calibration ranges and selected compound list as compared to methods developed for conventional cigarettes. The iQOS HEETS HTPs were compared against the 3R4F reference cigarette and we found that VOCs, aromatic amines, benzo[a]pyrene and TSNAs were reduced by 99 % and carbonyl compounds were reduced by 87 % when tested under intense smoking conditions.