In May 2016, the U.S. Food and Drug Administration (FDA) issued a final rule deeming cigar products within scope of their regulatory authority. Under this regulation the FDA will require manufacturers to report the quantities of Harmful and Potentially Harmful Constituents (HPHCs) in cigar filler and smoke. Currently, FDA has not indicated that the list of constituents for cigars would differ from that of the list of constituents provided for tobacco smoke, and thus would require the analysis of ammonia in cigar smoke. We previously showed that the CORESTA Recommended Method No. 83 for the analysis of ammonia in cigarette smoke may not be fit for purpose for all cigar types. We previously presented, at TSRC in 2017, data that shows amount of ammonia measured in cigar smoke depends on tobacco blend composition, and on the concentration of acid in the trapping solution. We also showed that ammonia is not stable in the final extracts, specifically, when stored at room temperature. Our current work is focused on improving our understanding of the formation of ammonia in cigar smoke to better understand the differences between cigars made with different tobacco blends. We have used Fourier-transform infrared spectroscopy (FTIR) and high resolution mass spectrometry to investigate the amount and potential source of ammonia measured in smoke from different cigars blends. In addition, we evaluated the trapping efficiency using ammonia gas to identify the optimal conditions for trapping. These results show that the ammonia gas is sufficiently trapped on the Cambridge Filter Pad and in the first impinger containing 0.1N H2SO4. We will also present data for ammonia for a range of market products with high burley content and with high dark air-cured tobacco content.