CORESTA Meeting, Agronomy/Phytopathology, 2015, Izmir, Turkey, AP 10

Biological control of Ralstonia solanacearum using Ralstonia phage and the influence of diversity of soil bacteria

WANG Fenglong(1); GAO Miao(1); QIAN Yumei(1); SHEN Lili(1); YU Guanghong(2); WANG Yaofeng(3); YANG Jinguang(1)
(1) Key Laboratory of Tobacco Pest Monitoring Controlling & Integrated Management / Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, Shandong, P.R. China; (2) Baoshan Oriental Tobacco Company Ltd, Baoshan, Yunnan, P.R. China; (3) Qingyang Company, Gansu Provincial Tobacco, Xifeng, Gansu, P.R. China

Tobacco bacterial wilt caused by Ralstonia solanacearum is one of the main diseases of tobacco. Ralstonia phage ∈RS-1 was isolated from the soil of tobacco bacterial wilt nurseries from experimental fields of the Tobacco Research Institute of CAAS Institute, Jimo District, Qingdao City. ∈RS-1 is an obligated parasite of R. solanacearum, and can infect and lyse R. solanacearum cells preventing them from dividing and reproducing and so controlling the disease pandemic. So ∈RS-1 has real potential as a new microbial inoculant on R. solanacearum infected fields. We irrigated the tobacco root with the ∈RS-1 culture (1×1010pfu/mL) before artificial inoculation of R. solanacearum. The result showed a significant decrease in disease severity when R. solanacearum was co-inoculated with ∈RS-1. The control efficiency of ∈RS-1 against R. solanacearum could reach 94.87%, and was higher than control by agricultural streptomycin. Additionally, the diversity of bacterial community in treated soil by ∈RS-1 was studied using metagenomic technique. The result showed that the soil R. solanacearum content is obviously lower than in the untreated control and ∈RS-1 does have remarkable influence to the other microorganisms. In treated soil, the quantity of bacteria showed a descending trend as well as actinomyces compared to the untreated control. So ∈RS-1 has the role of adjusting the diversity of soil bacteria.