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TSRC, Tob. Sci. Res. Conf., 2023, 76, abstr. 24

Carbonyl analysis of cigarette mainstream smoke by bag collection

HEARN B.(1); LEWIS E.(1); SHEPPARD C.(1); WATSON C.(2); VALENTIN L.(2)
(1) Battelle Analytical Services, Atlanta, GA, USA; (2) Center for Disease Control, Atlanta, GA, USA

Most previous methods for quantitatively determining carbonyl levels in mainstream cigarette smoke used impingers for sample collection. We present a simplified method using disposable Tedlar® bags, rather than impingers, to collect mainstream smoke. Conceptionally, we think of this as an “asynchronous disposable impinger” method because we perform in-bag derivatization after smoking. Results from our new approach shows that the bag contributes minimally to background or artifact formation and the derivatization reaction proceeds efficiently for all target analytes.

We compared results from our new approach to the older impinger methods to demonstrate equivalency. Parallel calibration curves were generated from the derivatization of the unreacted carbonyls in Tedlar bags as well as in glassware. The maximum slope difference between the curves for all analytes was equal to or lower than 9%.

Our new, robust method provided comparable results to prior existing methods that use impingers. All our carbonyl measurements for 3R4F and CM6 reference cigarettes fell within the reported confidence limits reported from a CORESTA 2012 collaborative study for both ISO and CI regimens. Similarly, seven of eight measurements of carbonyls for the 1R6F reference cigarette fell within the published limits from the University of Kentucky Center for Tobacco Reference Products Certificate of Analysis 1R6F (2016). Our simplified method produces less chemical waste, has higher throughput, and has a linear range suitable for measuring wide range of carbonyl yields from both ISO and Intense machine smoking regimens.

The findings and conclusions in this report are those of the authors and do not necessarily represent the official position of the CDC. Use of trade names is for identification only and does not imply endorsement by the CDC.