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Tobacco Science & Technology, 2011, 1, p. 36-42., ISSN.1002-0861

Caspase-mediated apoptosis of human bronchial epithelial cells induced by crotonaldehyde

LIU XING-YU(1,2); YANG ZHI-HUA(3); PAN XIU-JIE(3); ZHU MAO-XIANG(3); XIE JIAN-PING(4)
1. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning, P.R. China; 2. Beijing Work Station, Technology Center of Shanghai Tobacco (Group) Corporation, Beijing 101121, P.R. China; 3. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, P.R. China; 4. Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China

In order to assess the health risk of cigarette smoke, crotonaldehyde-induced cell death and its mechanism in BEAS-2B cells were investigated. Cell survival rate, intracellular ATP level, apoptosis rate, cytochrome c released from mitochondria to cytoplasm, and caspase-9 and caspase-3/7 activities were determined by MTT, chemiluminescence, flow cytometry, flow cytometry combined with immunochemistry, and chemiluminescence methods, respectively. The results showed that crotonaldehyde induced cell apoptosis, and turned into necrosis along with the increase of crotonaldehyde dosage. Crotonaldehyde caused that intracellular ATP level declined rapidly, mitochondria membrane potential decreased, cytochrome c released from mitochondria to cytoplasm, and caspase-9 and caspase-3/7 were activated. It was concluded that crotonaldehyde induced and caspase mediated cell apoptosis.