Bull. Spec. CORESTA Symposium, Taormina, 1986, p. 91, S03, ISSN.0525-6240

Characterization and identification of polycyclic aromatic hydrocarbons in cigarette smoke condensate by GC-MS in combination with retention indices

KLUS H.; BACHLECHNER G.; SCHMID E.R.
Austria Tabak, R&D, Vienna, Austria
Polycyclic aromatic hydrocarbons (PAH) were separated from cigarette smoke condensate (CSC) by liquid-liquid extraction, gel-chromatography, high performance liquid chromatography (HPLC), and capillary gas-chromatography (GC). Relatively pure PAH fractions were isolated fast and effectively by combining different HPLC columns (e.g. RP-2, RP-18) online. The PAH's were separated from each other by capillary GC using DB-5 as stationary phase. The PAH's were characterized by their retention times and especially by means of their retention indices (RI) according to Lee. Final identification was achieved in many cases by combining these RI _ data with results from gas-chromatography mass-spectrometry (GC/MS). E.g. isomers, showing the same mass-spectra, could be characterized by their RI's. The procedure was tested with 32 PAH standards and applied to CSC from Virginia tobacco. More than 50 PAH's were identified. Also quantitative determinations were carried out. To achieve this, response factors and recovery studies had to be done. Discrimination of the higher PAH's was observed during GC due to their low volatility.