CORESTA Meeting, Agronomy/Phytopathology, 2023, Cancun, APPOST 07

Chromosome-level genomes of the Nicotiana tabacum progenitors, Nicotiana sylvestris and Nicotiana tomentosiformis

SIERRO N.; OUADI S.; AUBERSON M.; DULIZE R.; GUEDJ E.; IVANOV N.V.; GOEPFERT S.
PMI R&D, Philip Morris Products S.A., Neuchâtel, Switzerland

As descendants of the Nicotiana tabacum progenitors, Nicotiana sylvestris and Nicotiana tomentosiformis are important diploid Nicotiana species. In addition, ancestors of N. sylvestris were involved in the polyploidization events that gave rise to the Suaveolentes and Repandae clades, reinforcing its importance within the Nicotiana species.

The genomes of these two Nicotiana species were published almost 10 years ago based on second-generation short-read sequencing, which resulted in fragmented scaffold-level assemblies. However, the fragmented nature of these assemblies and the lack of chromosome-anchoring limits their usability for synteny-based cross-species research.

Here we present de novo chromosome-level genomes for N. sylvestris and N. tomentosiformis built using primarily third-generation long-read sequencing, thereby bringing them to the completion level of other reference diploid Solanaceae genomes.


Philip Morris International is the sole source of funding and sponsor of this research.