CORESTA Meeting, Agronomy/Phytopathology, 2017, Santa Cruz do Sul, AP 29

Comparative proteomic analysis reveals differential protein and energy metabolisms from two tobacco cultivars in response to cold stress

HU Risheng(1); ZHU Xianxin(2); XIANG Shipeng(1); YANG Chengwei(3); LAI Jianbin(3); ZHANG Xianwen(2); LIU Zhi(2); ZHU Lieshu(2)
(1) Central South Agricultural Experiment Station of CNTC, Changsha, P.R. China; (2) Hunan Agricultural University, Changsha, P.R. China; (3) South China Normal University, Guangzhou, P.R. China

Given the development of tobacco is sensitive to low temperature, cold stress is a critical problem for tobacco production. Uncovering of the mechanisms for cold response in tobacco would contribute to improving its cold tolerance, but the precise molecular mechanism in this process remains unclear. In our novel screening for cold sensitivity in different tobacco cultivars, NC567 displayed a cold tolerance phenotype, while Taiyan8 is hypersensitive to low temperatures. In the current study, the samples of NC567 and Taiyan8, with or without cold treatment, were used in iTRAQ based proteomics to uncover their different mechanisms in response to cold stress. A total of 4317 distinct proteins were identified and the differential proteins in four comparison sets were used for further analysis. The GO analysis indicated that the majority of differentially expressed proteins were involved in metabolic and cellular processes. 55 proteins decreased in NC567 but increased in Taiyan8 under low temperature, while the levels of 42 proteins were lower in Taiyan8 at normal temperature but higher in Taiyan8 than NC567 under cold treatment, suggesting the variations for cold response in these cultivars. The levels of components involved in protein synthesis/degradation, photosynthesis/respiration, as well as ROS scavenging, were significantly different in these comparison sets, providing evidence for a model that protein metabolism and energy balance are essential for creation of an intracellular environment in response to low temperature in tobacco cells. In conclusion, our study identified several novel pathways associated with low temperature stress and provides hints for the further improvement of cold tolerance in tobacco plants.