The largest transpiration was observed in the hybrid with the most stomata, i.e. in the hybrid Drava which had mean daily Tr values of 4.48-12.56 mg/g/min (Bo zjakovina) and 3.58-10.51 mg/g/min (Pitoma ca), followed by Zagreb with Tr values of 3.91-9.26 mg/g/min Bo zjakovina) and 3.41-11.78 mg/g/min (Pitoma c), and by Podravina with Tr values of 4,60-10.03 mg/g/min (Bo zjakovina) and 3.37-8.80 mg/g/min (Pitoma ca). The highest mean Tr values were observed in the middle leaves (with the exception of Drava in the Pitoma ca location), and the lowest in the technologically mature bottom leaves and sometimes in the leaves just below the blossom (Zagreb in the Pitoma ca location and Podravina in both locations). The most intensive transpiration in the hybrids under investigation was observed from the beginning of flowering up to full bloom. Sufficient quantities of water should therefore be supplied in these phases of the plants development in order to achieve high yields . The hybrids Podravina and Zagreb, considering their transpiration capacity, are suitable for cultivation in relatively dry habitats.