Pseudomonas syringae pv. tabaci is the causal agent of angular leaf spot, a bacterial disease that affects dark tobacco. This bacterial disease has been the most significant foliar disease in dark tobacco production since 2015. Dark tobacco producers have relied on one bactericide to control angular leaf spot, Streptomycin. Lab studies conducted at the University of Kentucky, Research and Education Center in Princeton, KY have shown resistance to Streptomycin at the 200 ppm field rate. Since 2015, 113 isolates have been screened for resistance to streptomycin and 25% (28) of those isolates have shown resistance at the 200 ppm field rate. Field spray trials have been ongoing since 2015, at the University of Kentucky, Research and Education Center in Princeton, KY and at the West Farm of Murray State University in Murray, KY. Spray trials were established at both locations in 2021 to evaluate direct and plant-mediated inhibitory effects of eight different chemical products. Antibiotic, biocontrol and/or synthetic bactericide products were evaluated at the Princeton location, and the evaluation of copper products as alternatives to streptomycin were conducted at the Murray location. Products included in both spray trials are either labeled or have the potential to be labeled for dark tobacco. Plots were four rows wide, 40 ft. long by 13 ft. in wide, with a row spacing of 40 in. and in-row plant spacing of 32 in. (4,900 plants/ac). Both trials were inoculated with a streptomycin sensitive strain of P. syringae pv. tabaci at 10⁷ cf/ml at 30 psi. Plant disease rating were taken three times during the growing season at both locations, disease pressure continued to increased overtime at both locations. There were no significant differences in yield and grade quality index at either location. Although, at the Murray location, all treatments yielded higher than the untreated control. Current recommendations for dark tobacco producers is to continue utilizing streptomycin in field with sensitive isolates, and integrate the use of copper products and surface sterilants in fields with resistance isolates. (Reprinted with permission)