TSRC, Tob. Sci. Res. Conf., 2019, 73, abstr. 034 (also presented at CORESTA SSPT2019)

Comparison of a flame ionization detector (GC/FID) to a nitrogen-phosphorus detector (GC/NPD) for gas chromatographic determination of nicotine in conventional and ultra-low nicotine tobacco blends

Liggett Group, Mebane, NC, USA

The established CORESTA method to determine nicotine in tobacco uses a gas chromatograph coupled with a flame ionization detector (GC/FID) (CRM 62) or that uses a mass spectrometer (GC/MS) (CRM 87). These methods were developed to evaluate conventional tobacco. Currently, there are no standardized methods available to quantitate the nicotine level in both conventional and in ultra-low nicotine tobacco. Other selective instruments such as a gas chromatograph coupled with a nitrogen-phosphorus detector (GC/NPD), high performance liquid chromatography coupled with an ultraviolet detector (HPLC/UV) and a gas chromatography–mass spectrometer (GC-MS) have been reported for conventional tobacco but have not been widely reported for the evaluation of ultra-low nicotine tobacco. In the study we will compare the nicotine results between a GC/FID and GC/NPD systems using an ultra-low nicotine tobacco blend (NIST SRM 3222), CRP1.1, conventional mid-level tobacco leaf, and a conventional tobacco blend (1R6F). We will present instrument precision, method precision, accuracy and LOD & LOQ of both methods. We will also present the advantages and disadvantages of the two detectors.