The U.S. Alcohol and Tobacco Tax and Trade Bureau (TTB) is responsible for determining proper tax classification of tobacco products. Tobacco products in the U.S. may fall into several taxable categories including cigars, cigarettes, snuff, chewing tobacco, pipe tobacco and roll-your-own. As major components of tobacco, carbohydrates are valuable for product characterization and differentiation. The p-hydroxybenzoic acid hydrazide (PAHBAH) method is widely used for the determination of carbohydrates, specifically reducing sugars, in tobacco. It was adapted to Segmented Flow Analysis (SFA) as a high throughput application and is available commercially though several manufacturers as an analytical cartridge. Ion chromatography with pulsed amperometric detection (IC-PAD) is also widely utilized for the determination of carbohydrates. IC separations of glucose, fructose, and sucrose are achieved using a Dionex CarboPac PAl analytical column. It is more selective than the SFA method but does not share its high throughput capabilities. LC-MS/MS is also used as a technique for quantification of carbohydrates in tobacco extracts. LC-MS/MS separations of glucose, fructose, and sucrose are achieved using a LC-NH2 column. Negative ion electrospray with multiple reactions monitoring (MRM) mode is utilized for detection. Of the three methods presented here, LC-MS/MS appears to be the most selective. Results obtained from the analysis of tobacco and tobacco products using SFA, IC-PAD, and LC-MS/MS will be presented for comparison and differences in the selectivity of these methods will be discussed.