A comparison of two different extraction methods for aromatic amines in mainstream smoke using gas chromatography-tandem mass spectrometry (GC/QQQ)
The established GC-MS methods for the analysis of aromatic amines (o-Anisidine, o-Toluidine, 2,6-Dimethylaniline, 1-Aminonaphthalene, 2-Aminonaphthalene, and 4-Aminobiphenyl) involve complex sample preparation and analyzing the samples on a gas chromatograph with a single-quadrupole mass spectrometer. In this study, we have elected to use a triple-quadrupole mass spectrometer that greatly improves the selectivity of detection of the compounds of interest.
The objective of this study was twofold: (1) compare the establish two-step solid phase extraction (SPE) method verse a one-step extraction method, and (2) compare the results using a triple-quadrupole mass spectrometer under electron impact ionization, a triple-quadrupole mass spectrometer using chemical ionization and the established single-quadrupole method (GC-MS).
We will present our finding that the triple-quadrupole mass spectrometer enables simplification of the extraction method and offers superior limits of detection compared to the quadrupole mass spectrometer based standard method.
We will present instrument/method precision, accuracy and limit of detection (LOD) and limit of quantitation (LOQ) of both methods.