CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, STPOST 40 (also presented at TSRC 2017)

A comparison of two different extraction methods for aromatic amines in mainstream smoke using gas chromatography-tandem mass spectrometry (GC/QQQ)

STINSON A.(1); GILLILAND S.(1); HUCKINS A.(1); GILLMAN I.G.(2); BROWN S.S.(2); STEELMAN D.(1)
(1) Liggett Group LLC, Mebane, NC, U.S.A.; (2) Enthalpy Analytical Inc., Durham, NC, U.S.A.

The established GC-MS methods for the analysis of aromatic amines (o-Anisidine, o-Toluidine, 2,6-Dimethylaniline, 1-Aminonaphthalene, 2-Aminonaphthalene, and 4-Aminobiphenyl) involve complex sample preparation and analyzing the samples on a gas chromatograph with a single-quadrupole mass spectrometer. In this study, we have elected to use a triple-quadrupole mass spectrometer that greatly improves the selectivity of detection of the compounds of interest.

The objective of this study was twofold: (1) compare the establish two-step solid phase extraction (SPE) method verse a one-step extraction method, and (2) compare the results using a triple-quadrupole mass spectrometer under electron impact ionization, a triple-quadrupole mass spectrometer using chemical ionization and the established single-quadrupole method (GC-MS).

We will present our finding that the triple-quadrupole mass spectrometer enables simplification of the extraction method and offers superior limits of detection compared to the quadrupole mass spectrometer based standard method.

We will present instrument/method precision, accuracy and limit of detection (LOD) and limit of quantitation (LOQ) of both methods.