TSRC, Tob. Sci. Res. Conf., 2017, 71, abstr. 048 (also presented at CORESTA SSPT2017)

A comparison of two different extraction methods for aromatic amines in mainstream smoke using gas chromatography-tandem mass spectrometry (GC/QQQ)

STINSON A.(1); GILLILAND S.(1); HUCKINS A.(1); BROWN S.S.(2); GILLMAN I.G.(2)
(1) Liggett Group, Mebane, NC, USA; (2) Enthalpy Analytical, Durham, NC, USA

The established GC-MS methods for the analysis of aromatic amines (o Anisidine, o Toluidine, 2, 6 Dimethylaniline, 1 Aminonaphthalene, 2 Aminonaphthalene, and 4 Aminobiphenyl) involve complex sample preparation and analyzing the samples on a gas chromatograph with a single-quadrupole mass spectrometer. In this study, we have elected to use a triple-quadrupole mass spectrometer that greatly improves the selectivity of detection of the compounds of interest.

The objective of this study was twofold: (1) compare the establish two –step SPE extraction method verse a one-step extraction method and (2) compare the results using a triple-quadrupole mass spectrometer under electron impact ionization, a triple-quadrupole mass spectrometerr using chemical ionization and the established single-quadrupole method (GC-MS).

We will present our finding that the triple-quadrupole mass spectrometer enables simplification of the extraction method offers superior limits of detection compared to the quadrupole mass spectrometer based standard method. We will present instrument/ method precision, accuracy and LOD & LOQ of both methods.