In order to study the delivery behavior of ammonia and its correlation with routine analytes in mainstream cigarette smoke, including tar, nicotine and moisture content, 8 cigarette samples of different specifications and price categories were tested. The contents of ammonia, tar, nicotine and moisture content in particulate matters were determined respectively puff-by-puff; and the correlation of ammonia with tar, nicotine and moisture deliveries was analyzed. The multiple linear regression equation was developed. The results showed that: 1) Ammonia delivery increased linearly puff by puff, which was extremely significantly positively correlated to the contents of tar and nicotine and negatively correlated to moisture content. 2) The developed regression equation fitted pretty well.