Determination of 11 polycyclic aromatic hydrocarbon biomarkers in human urine with LC-MS/MS
In order to evaluate the exposure to PAHs in environmental tobacco smoke, the influences of solid phase extraction column, chromatographic column and mass spectrometry conditions on analytical results were investigated, a method with SPE-HPLC/MS/MS was established to determine the biomarkers of 1-OHNap, 2-OHNap, 1-OHPyr, 6-OHChr, 3-OHFlu, 1-OHPhe, 2-OHPhe, 3-OHPhe, 4-OHPhe, 9-OHPhe and 2-OHFlu in human urine. Using 1-OHNap-d8, 3-OHPhe-d9 and 1-OHPyr-d9 as internal standards, the samples were hydrolyzed by β-glucosidase/aryl sulfatase, extracted by Bond Elut C18 cartridge, separated by Eclipse PAH column, determined by mass spectrometry under multi-reaction monitoring mode (MRM), the OH metabolites of PAHs (OHPAHs) in urine were detected. The results showed that the limits of quantification (LOQ) of OHPAHs varied from 6 to 130 pg/mL. The detectable concentration of OHPAHs showed good linear relationship with r from 0.9991 to 1.0000, and the recoveries ranged from 72.45 % to 124.50 %. The intra-day and inter-day precisions were in the range of 1.49 % to 7.35 % and 2.90 % to 12.00 %, respectively. This method is suitable for simultaneously determining the biomarkers of PAHs in human urine.