Tobacco Science & Technology, 2012, 4, p. 37-43, ISSN.1002-0861

Determination of 11 polycyclic aromatic hydrocarbon biomarkers in human urine with LC-MS/MS

CHEN Yusong; WANG Sheng; YU Jingjing; LIU Huimin*; CAO Depo
Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China

In order to evaluate the exposure to PAHs in environmental tobacco smoke, the influences of solid phase extraction column, chromatographic column and mass spectrometry conditions on analytical results were investigated, a method with SPE-HPLC/MS/MS was established to determine the biomarkers of 1-OHNap, 2-OHNap, 1-OHPyr, 6-OHChr, 3-OHFlu, 1-OHPhe, 2-OHPhe, 3-OHPhe, 4-OHPhe, 9-OHPhe and 2-OHFlu in human urine. Using 1-OHNap-d8, 3-OHPhe-d9 and 1-OHPyr-d9 as internal standards, the samples were hydrolyzed by β-glucosidase/aryl sulfatase, extracted by Bond Elut C18 cartridge, separated by Eclipse PAH column, determined by mass spectrometry under multi-reaction monitoring mode (MRM), the OH metabolites of PAHs (OHPAHs) in urine were detected. The results showed that the limits of quantification (LOQ) of OHPAHs varied from 6 to 130 pg/mL. The detectable concentration of OHPAHs showed good linear relationship with r from 0.9991 to 1.0000, and the recoveries ranged from 72.45 % to 124.50 %. The intra-day and inter-day precisions were in the range of 1.49 % to 7.35 % and 2.90 % to 12.00 %, respectively. This method is suitable for simultaneously determining the biomarkers of PAHs in human urine.