TSRC, Tob. Sci. Res. Conf., 2021, 74, abstr. 46

Determination of 2-Chloro-1,3-propanediol (2-mcpd) and 3-Chloro-1,2-propanediol (3-MCPD) in emissions by gas chromatography-mass spectrometry (GC-MS)

Labstat International Inc., 262 Manitou Dr., Kitchener, Ontario, Canada

Monochloropropanediol (MCPD) and glycidol are thermally generated toxicants found in refined vegetable oils and glycerin. The purpose of this study was to develop a method for the quantitation of 2-Chloro-1, 3-propanediol (2-MCPD) and 3-Chloro-1,2-propanediol (3-MCPD) in the emissions from various nicotine and glycerin containing products.

Total particulate matter (TPM), or the aerosol collected mass (ACM) equivalent, is collected on a glass fibre filter pad. Internal Standard (ISTD) solution containing 2-MCPD-d5 and 3-MCPD-d5 is added to the pad and extracted with isopropyl alcohol (IPA). An aliquot of the extract is combined with a phenylboronic acid (PBA) solution in diethyl ether to form the PBA derivatives (MCPD-PBA). The sample is concentrated to dryness and reconstituted in isooctane. The samples analyzed by GC-MS using a DB-5 UI 60m x 0.25mm x 0.25µm fused silica capillary column and selected ion monitoring (SIM) data acquisition mode. Quantitation is achieved through internal standard calibration.

A common set of calibration standards ranging from 40 to 10000 ng/mL is used for the combustibles and heated tobacco products emission matrices. E-vapour products are analyzed using a calibration range from 12 to 40 ng/mL. Trapping efficiency studies performed by adding impingers containing IPA showed no detectable levels of either 2-MCPD or 3-MCPD in the vapour phase. Recoveries of 2-MCPd and 3-MCPD using fortified samples of the KR 1R6F ranged from 87 to 116% and 97.5 to 114% respectively, showing excellent accuracy across a range of fortified levels.