The determination and comparison of hydrogen cyanide (HCN) levels in the mainstream smoke of three reference cigarettes using three different analytical techniques to understand differences and variability between methods
The FDA Center for Tobacco Products has identified hydrogen cyanide (HCN) as a harmful and potentially harmful constituent (HPHCs) in tobacco smoke. Currently there is no internationally accepted method for the analysis of hydrogen cyanide but at least one national health organization has adopted the use of continue flow analyzers (CFAs) for the determination of hydrogen cyanide content of tobacco smoke. Several other analytical techniques are also commonly used to measure HCN including Ion Chromatography (IC) with electrochemical detection and spectrophotometric detection. In this presentation, we will compare the hydrogen cyanide concentration determined by each method in the mainstream smoke from 3R4F, CM7 and 1R5F reference cigarettes under both ISO and Canadian Intense smoking regimes and compare the advantages and disadvantages of each. All measurements were made from the same set of smoke samples collected at Liggett Group; Liggett conducted CFA analysis, and Enthalpy Analytical analyzed the samples using a spectrophotometric method and using IC with electrochemical detection. This study was designed to minimize sample differences since each test method was performed on the same sample. We will present the analytical values for each assay, short-term repeatability, and compare the results of the three methods used to determine HCN in mainstream smoke.