Tobacco Science & Technology, 2011, 7, p. 43-47., ISSN.1002-0861

Determination of fifteen pesticide residues in tobacco with LC-MS/MS

YAN HUI-HUI(1); HU BIN(2); LIU HUI-MIN(2); MA HAI-MING(1); WANG LI-JIE (1); SHI JIE(1)
1. Department of Chemistry, Zhengzhou University, Zhengzhou 450001, P.R. China; 2. Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China

A method for rapidly determining fifteen pesticides, such as pymetrozine and others, in tobacco with LC-MS/MS was developed. Samples were extracted by acetonitrile, cleaned up by dispersive solid-phase extraction and finally detected with LC-MS/MS. The results showed that the recoveries of the fifteen pesticides ranged from 70.43% to 117.81% at the standard’s levels of 0.1, 0.25 and 0.5 μg/g, the relative standard deviation (RSD) ranged from 1.16% to 13.89%, and the limit of detection was from 0.004 to 0.030 μg/g. This method was suitable for the rapid analysis of multiple pesticide residues in batches of tobacco samples.