Tobacco Science & Technology, 2012, 4, p. 28-32, ISSN.1002-0861

Determination of hydrogen peroxide in mainstream cigarette smoke by highly selective fluorescence method with Amplex Red/HRP

PENG Shuhai(1); ZHANG Ying(2); LIU Hailin(1); ZHUANG Yadong(2); JIANG Chengyong(1); SUN Shihao(1); ZONG Yongli(1); XIE Jianping(1)
(1) Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China; (2) Technology and Research Center, China Tobacco Jiangsu Industrial Co., Ltd., Nanjing 210093, P.R. China

A highly selective fluorescence method with Amplex Red HRP was developed to determine the hydrogen peroxide in mainstream cigarette smoke. This method’s LOD=13.5 nmol/L, recovery 98.3 %-101.7 % and RSD<3.17 % (n=5). The level of hydrogen peroxide in mainstream smoke was of micrograms per cigarette and positively correlated with tar delivery. For the cigarettes with the same tar delivery, the delivery of hydrogen peroxide was dependent on their design parameters. This method is suitable for determining the delivery of H2O2 in mainstream cigarette smoke.