Bull. Spec. CORESTA Congress, Harare 1994, p. 183, S 6

Determination of organic acids in cigarette smoke by HPLC and capillary electrophoresis

SAINT-JALM Y.; LAGOUTTE D.; LOMBARD G.; NISSERON S.; PAPET M.P.
SEITA, Centre de Recherche, Fleury les Aubrais, France
Two methods for the determination of the major organic acids in cigarette smoke (formic, acetic, lactic and glycolic acids) were developed in this study : - an HPLC method with pre-column derivatization of the acids through esterification with para-bromophenacyl bromide. - a direct analysis method using capillary electrophoresis (CE). A comparison of both methods demonstrates that they give identical results. Repeatability of injection and smoking in both methods were evaluated. Variation coefficients ranged from 6 to 12.7% for the HPLC method and from 2.8 to 12.4% for the CE method when injection was repeated. Variation coefficients ranged from 4.2 to 11% for the HPLC method and from 1.2 to 14% for the CE method when smoking was repeated. Detection limit, calculated for a signal/noise ratio of 3, was approximately 10-6 mol/l for all acids and for both methods. This limit corresponds to the injection of 5 pmole acid for the HPLC method and 0.5 pmole for the CE method. The capillary electrophoresis method, which calls for no special preparation of the sample, combined with a short length of time for analysis (15 min), appears to be today's optimal method for the routine determination of organic acids in smoke.