Tobacco Science & Technology, 2010, 10, p. 37-40., ISSN.1002-0861

Determination of phosphate content in tobacco with continuous flow method

ZHANG WEI(1); WANG YING(1); YU RUI-GUO(2); TANG GANG-LING(1); XU RU-YAN(3); BO YUN-CHUAN(3); LI RONG(2)
1. China National Tobacco Quality Supervision & Test Center, Zhengzhou 450001, P.R. China; 2. Guangzhou MilliM Herbal Extract Technology & Development Inc., Guangzhou 510663, P.R. China; 3. Xuzhou Cigarette Factory, China Tobacco Jiangsu Industrial Corporation, Xuzhou 221005, Jiangsu, P.R. China

A continuous flow method for determining the phosphate content in tobacco was developed. The tobacco samples were extracted with deionized water, using ammonium molybdate and ascorbic acid solution as a colorific agent and potassium antimony tartrate as a catalyst. The results indicated that the average recoveries of phosphate ranged from 98.3% to 101.2% with the RSD of 1.9% to 2.1% and the reproducibility of below 0.02%.