We have created a series of DNA promoters useful for expressing foreign genes in transgenic plants. We have also designed and constructed a small and highly efficient binary Ti vector, pSiM24, for transformation of higher plant cells. We reduced the size of the backbone of and earlier binary vector, pKYLXM24 (GenBank Accession No. HM036220), a derivative of pKYLX71 (Schardl et al., 1987) from 12.8 Kb to 7.1 kb. The binary vector pSiM24 is composed of modified genetic elements. The pSiM24 plasmid offers a wide selection of cloning sites, a high copy number in Escherichia coli, and high cloning capacity for easily manipulation of different genetic elements. The vector has been fully tested in transferring transgenes (GFP and GUS) both transiently (Agro-infiltration, protoplast electroporation, and biolistics), and stably in plant systems (Arabidopsis and tobacco). Using both Agrobacterium and biolistic procedures, this vector has been tested to express a real gene like IL10; hence pSiM24 would be useful for nuclear transformation as well as plant made product (PMP) applications (Sahoo et al., 2014. PLoS ONE 9(6): e98988. doi:10.1371/journal.pone.0098988). In addition, the pSiM24 plasmid can act as a platform for various applications like gene expression studies and different promoter expression analyses. The sequence information for pSiM24 and relevant genetic elements is provided in NCBI database under GenBank accession no. KF032933. We are modifying this plasmid to express Cas9 gene and specific sgRNAs to edit target genes in plant genomes.