TSRC, Tob. Sci. Res. Conf., 2016, 70, abstr. 16

Development and evaluation of a benchtop walkaway platform for the automated solvent extractions of cambridge filter pads and agricultural tobacco samples

LI Shijie(1); LIU Wei(1); COX J.(2); LU J.(2)
(1) China Tobacco Yunnan Industrial Co., Ltd, Kunming, China; (2) Sirius Automation Inc., Buffalo Grove, IL, USA

Several documented methodologies require liquid / solid extraction of smoking machine residues from standard size Cambridge filters. The efficiency and reproducibility of such solvent extractions is paramount for optimal results hence the need for operator- independent techniques, and/or appropriate automation. The requirement for multiple/ mixed solvents, variable volumes, choices in vessel types, agitation time and single/multiple sampling and potential ‘disposable disc’ filtration options must be factored into the project design. Operator preferences to select protocols for wash steps/multiple washes are also essential.

Researchers seeking to analyze cryogenically ground tobacco leaf samples have similarly stringent demands. Our poster describes the development and usage of an automated Extraction Station based around standard and customary approaches, but utilizing a robotic platform to ensure Uniform Sample History, and an ongoing chain-of-operations database for all samples thus prepared.

Extraction efficiencies and %RSDs will be presented and compared with manual approaches, as well as sample numbers and throughput/time. An examination of improvements requested by users will also be offered.