Specific heterocyclic aromatic amines (HAAs) are on the full Hoffmann analytes list and are considered highly mutagenic. These compounds are pyrolytic products of amino acids or proteins and have been reported in cooked meat, diesel exhaust and tobacco smoke condensate. Reported here is a simple procedure applicable to the quantitative determination of several HAAs, including AaC, MeaC, IQ, Trp-P-1, Trp-P-2, Glu-P-1, Glu-P-2, and PhIP, as well as MeIQ and MeIQx. A liquid chromatography-triple-quadrupole tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the quantitative determination of HAAs in mainstream smoke condensate. Unlike previously reported methodology, this procedure does not employ solid phase extraction (SPE) cleanup and pre-concentration, thereby decreasing cost while increasing sample throughput. A longer HPLC analysis time was used to provide better chromatographic separation of the HAAs on the LC column with the minimized sample preparation. The HAAs were extracted with methanol from a 44 mm Cambridge filter pad containing mainstream cigarette smoke condensate from 2-5 cigarettes, depending on smoking regime intensity. The extract was filtered then diluted 12 fold with more methanol. This diluted sample was then injected onto the HPLC coupled with a triple-quadrupole tandem mass spectrometer equipped with electrospray ionization (ESI). AC18 (ODS) column was used for the separation. HAAs were characterized using product ions selected during direct infusion of individual stock standard solutions into the mass spectrometer LC mobile phase flow. Quantitative analysis was performed using 4,8-DiMeIQx as internal standard. The accuracy of this procedure was determined by standard addition experiments, which showed average recoveries of most HAAs between 98-119%, except PhIP at 89%.