CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, STPOST 23

Dosimetry: the effects of cigarette smoke dilution on nicotine delivery in a 24 and 96 well format using Smoke Aerosol Exposure In Vitro System (SAEIVS)

BODE L.M.(1); ROEWER K.(1); OTTE S.(1); WIECZOREK R.(1); SIMMS L.(2)
(1) Reemtsma Cigarettenfabriken GmbH (an Imperial Brands PLC Company), Hamburg, Germany; (2) Imperial Tobacco Limited, Bristol, U.K.

In vitro aerosol exposure systems are useful tools for screening of potential toxic effects of freshly generated smoke of conventional cigarettes[1]. An in house platform “Smoke Aerosol Exposure In Vitro System (SAEIVS)” (assembled by Burghart TABAKTECHNIK) was used to generate fresh whole smoke to simulate exposure of cells at the air liquid interface (ALI)[2].

The aim of this study was to increase our knowledge of the effects of smoke dilution on delivery of nicotine to an in vitro system. A CORESTA monitor test piece, CM7, was smoked under ISO 3308 smoking conditions to deliver smoke to 24 or 96 well multi titer plate (MTP) formats. The surface of wells in MTP contained (300/50 µl) of PBS to simulate the wet surface of the ALI exposure model.

A puff gradient was used every time in 2 puff-step-wise. All experiments were performed in three replicates. The method used to measure nicotine was validated for selectivity and sensitivity LC-MS/MS (AB Sciex Qtrap 2000). Nicotine was quantified by two specific mass transitions (Quantifier and Qualifier) with an internal Standard (Nicotine-d4).

In this poster presentation, the validation process and necessary validation parameters, e.g. precision, repeatability, recovery will be discussed in relation to the effects of smoke dilution on nicotine deposition in 24 and 96 well plates.

[1] Thorne D.; CORESTA Congress, Berlin, 2016, Smoke Science/Product Technology Groups, STPOST 26
[2] Wieczorek R.; CORESTA Meeting, Smoke Science/Product Technology, 2015, Jeju, ST 44