Applications of chloride (Cl^-) to tobacco are generally discouraged due to the negative effects the anion can have to yield, quality, and smoke flavor/aroma. Despite the negative effects associated with Cl^- application, unintentional applications of the nutrient sometimes occur. The season-long implications of Cl^- are not well known. Research was conducted in North Carolina to evaluate the impact of four Cl^- application rates (0, 33.6, 67.3, and 100.9 kg Cl^- /ha) when liquid urea-ammonium-nitrate was used as the sole source of applied nitrogen. Tissue samples were collected at five growth stages: two weeks after fertilizer application, at layby, two weeks after layby, at topping, and after curing. Leaf Cl^- concentration increased with application rate, with the 67.3 and 100.9 kg rates typically being greater than the 1% threshold established by industry. Despite high Cl^- application rates, toxicity symptoms were not observed, thus indicating that visual symptoms of excessive Cl^- cannot be solely relied upon to determine if misapplication has occurred. Leaf yield, quality, and value were not affected by Cl^- application; although, it is probable that smoke flavor and aroma may be negatively impacted at application rates greater than 33.6 kg Cl^- /ha. (Reprinted with permission)