To evaluate the effect of drought stress in different growth stages on quantitative and qualitative yields, of Virginia tobacco, the experiment was carried-out in CRD with 8 treatments and 3 replications in potty conditions for two years (2004-5). Three stages were:1- until 30 days after transplanting, 2- from 31 days after transplanting to flowering and 3- from flowering to end of growth stage. Treatments were: (1) stress in three stages; (2) stress in stages 1 and 3; (3) stress in stages 2 and 3; (4) stress in stage 2; (5) non -stress in three stages; (6) stress in stages 1 and 2; (7) stress in stage 3, and (8) stress in stage 1. The results showed that there were significant differences on 1% level for yield, income, plant height and on the 5% level for length leaf, price of 1 kilo and sugar percent. The greatest and lowest yields belong to the 8^th treatment with 92.55 g/plant and the 3^rd treatment with 51.5 g/plant respectively. Second treatment with 1.51 g/litre had more water use efficiency. The results of combined analysis for two years showed that all factors affected by year except water use efficiency, income and price of 1 kilo. But the interaction effect between year and treatments showed that factors had same process in two years. According to results, irrigation during the first 30 days after transplanting when irrigation is not possible in other stages is not useful because irrigation in this period causes more growth of stem and less growth in roots. But non-irrigation in first 30 days after transplanting and irrigation after this time gives higher yield. As water deficiency at the first stage increases root growth yet limits stem growth, the plant can use water in other stages more efficiently.