Two-year (1991-1992) comparative trials were carried out with oriental genotypes Prilep 12-2/1 (standard), Prilep 156/1 and Prilep 7. The trials consisted of three variants, one of which served as control (untreated), and two variants of laser stimulation (L₁ and L₂), of the seedlings prior to transplanting. Chemical analyses of tobacco raw obtained from the above mentioned genotypes with their laser variants were made according to standard methods. It could be stated from the results that chemical characteristics of tobacco raw vary in relation to the genotype, climate conditions and laser stimulation. Thus, the average nicotine content in the investigated genotypes ranged from 0,82% to 1,54% for Prilep 12-2/1 and Prilep 7 respectively. The protein content was lowest in Prilep 7 (7,29%) and highest in the standard Prilep 12-2/1 (7,54%). Soluble sugars average 18,93% (Prilep 12-2/1) to 20,69% (Prilep 156/1). The optimum (high) content of soluble sugars was recorded in 1992, when the amount of precipitations was high. The new genotypes have a lower average content of mineral matters as compared to the standard, ranging from 10,00% to 14,79% in Prilep 156/1 and P 12-2/1, respectively. Shmuk's number, which indicates the quality of tobacco, is relatively high and ranges 2,95 to 3,08 in Prilep 12-2/1 and Prilep 7, respectively. The laser stimulation has a positive effect on chemical properties of tobacco. It relatively decreases the nicotine content to 13,18% (L₁) and 23,17% (L₂); proteins to 16,98% (L₁) and 16,60% (L₂) and mineral matters to 15,82% (L₁) and 7,86% (L₂). The laser stimulation increases the soluble sugars content to 12,63% (L₁) and 22,25% (L₂) and the values of the Shmuk's number to 12,67% (L₁) and 48,33% (L₂).