For investigating the influence of drought stress on the growth, physiological and biochemical characteristics of transplanted seedlings cultured in different seedbed systems, the pot trials with controlled soil moisture content were conducted to study the seedbed systems for flue-cured tobacco seedlings adaptable to drought conditions. The results showed that the proline content and POD activity in tobacco plants presented a rising trend under drought stress. With the increase of duration and degree of drought, the activity of NR in tobacco plants decreased. Under the condition of moderate drought, the growth status of transplanted tobacco seedlings from float-moist seedbed was better than that from floating system; while under extremely drought condition, the growth of tobacco plants from both two seedbed systems stagnated, yellowing and wilting leaves emerged 14 days after transplanting.