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47th TWC, Tob. Work. Conf., 2016, abstr. 25

Evaluation of androgenic and gynogenic doubled haploid lines for use as parental lines for hybrid burley tobacco varieties

MILLER R.D.; DEOLIVEIRA E.
University of Kentucky, Lexington KY USA

Ten androgenic haploid (ADH) lines and ten gynogenic haploid (GDH) lines, developed via anther culture and an interspecific cross with Nicotiana africana, respectively, were derived from the inbred burley tobacco (Nicotiana tabacum L.) cultivar TN 90LC. Eight ADH and four GDH lines were also derived from burley parental line GR 149LC. Mid-vein culture was then utilized to double the chromosome number of each haploid line. For TN 90LC, each of the ten ADDH and ten GDDH doubled haploid lines were randomly paired with TN 90LC to form a triplet. The ten TN 90 triplets were evaluated for agronomic traits at three locations utilizing a randomized complete block with three replications. The GR 149LC ADDH and GDDH lines were evaluated in a similar fashion. For TN 90LC, on average the ADDH lines yielded 3.9% less and the GDDH lines yielded 1.9% more than the inbred source. For GR 149LC, the average yields of the ADDH lines, GDDH lines, and inbred source were 3357, 3343, and 3351 Kg/ha, respectively. Individual ADDH and GDDH lines that produced yields equal to or greater than their inbred source were identified for both TN 90 and GR 149. Each of the ADH and GDH TN 90 lines was crossed with TKS 2002LC, the female parental line of the hybrid variety KT 204LC. Similarly, each of the GR149 ADH and GDH lines was crossed with ms TN 90LC, the female parental line of the hybrid variety TN 97LC. The hybrid varieties were then evaluated in the same manner described for the parental lines. For both the KT 204LC and TN 97LC families, no yield differences were observed in hybrids having either ADDH or GDDH lines as the male parent in comparison to hybrids having the original inbred line as the male parent. (Reprinted with permission)