Bull. Spec. CORESTA Congress, Jerez de la Frontera, 1992, p. 93, ST19, ISSN.0525-6240

Evaluation of Millipore's methoprene Envirogard TM ELISA kit

Philip Morris USA, Richmond, VA, USA
More and more frequently, classical analytical laboratories are implementing enzyme-linked immunosorbent assays (ELISAs) to facilitate the routine quantitative analysis of pesticide residues in food samples, for monitoring drinking water contamination an for monitoring the application of crop protection agents. For example, Millipore has recently introduced two ELISA based assays for the evaluation of methoprene levels on treated tobacco. These assays, their EnviroGard (TM) Methoprene plate and Tube kits, are designed for the quantitative and semi-quantitative analyses of the levels of methoprene on treated tobacco, respectively. We have evaluated the kits for ease of use, reproducibility, and variance of analyses compared with a traditional HPLC-based assay (1) currently used in our analytical division. For that evaluation, 5 methoprene treated tobacco samples, prepared with target levels of 0, 1, 5, 10, and 20 ppm methoprene, were analyzed in triplicate on 5 separate days using the plate kit, the tube kit and the HPLC based methoprene assays. This presentation will discuss the results from that evaluation. (1) Yang S.S., A rapid method for the determination of methoprene in tobacco by high performance liquid chromatography, Chromatographia, in press.