In order to reduce the undesirable components in paper-making process reconstituted tobacco, the effects of adding pectinase, hemicellulase and protease in tobacco fines and stems extraction, adding rates, enzymatic hydrolysis temperature and time on the contents of protein, holocellulose and pectin in the extracted tobacco fines and stems, and contents of reducing sugar and amino acid in the extract were studied. The results showed that: 1) the appropriated extraction conditions were: extraction temperature 50 °C for 2 hours in the presence of 0.5% (w/w) enzyme mixture (mass ratio of pectinase, hemicellulase and proteinase was 2:2:1) for tobacco stem, and 0.8%(w/w) enzyme mixture (mass ratio of pectinase, hemicellulase and proteinase was 1:2:2) for tobacco fines. 2) Comparing with hot water extraction, the enzymatic extraction increased the hydrolysis rates of protein, holocellulose and pectin in tobacco stems and tobacco fines by 7.63, 4.97, 15.87 and 16.59, 5.58, 16.13 percentage points, respectively; the reducing sugar in the enzymatic extract increased by 2.55 and 3.68 percentage points, while the viscosity of the enzymatic extract decreased by 0.32 and 0.149 Pa•S, respectively.