Pseudomonas syringae pv. tabaci is the causal agent of angular leaf spot, a bacterial disease that affects dark tobacco. This bacterial disease has been the most significant foliar disease in dark tobacco production since 2015. The antibiotic streptomycin has been the only chemical control recommendation for angular leaf spot. Lab studies conducted at the University of Kentucky, Research and Education Center in Princeton, KY have indicated resistance to Streptomycin at the 200 ppm field rate. Since 2015, 113 isolates have been screened for resistance to streptomycin and 25% (28) of those isolates have shown resistance at the 200 ppm field rate.  In 2020, an observational study was established to monitor angular leaf spot incidence in grower’s fields. The objective of this study was to find a correlation between both environmental and non-environmental management factors and presence of angular leaf spot. Included in this study were 30 dark tobacco fields located across seven counties in western Kentucky and Tennessee. Growers were not asked to alter management plans throughout the growing season, all major management operations were recorded. Fields were visited three times during the growing season. Seventeen different environmental and management variables were observed throughout the growing season. Three plots (two rows wide, by 40 ft. long) were designated in each field to represent the field’s topography and characteristics. At each location a soil sensor was placed 3 in. below the soil surface to measure soil temperature, a light meter was placed 12 in. above ground to measure light interception, both placed in the plant row. In 2020, fields with documented angular leaf spot received a rain gauge at the second field visit. In 2021, all 30 field received a rain gauge at the first field visit, to measure rainfall throughout the entire growing season. In 2020, there were four of 30 fields with confirmed angular leaf spot, one field having resistant isolates to Streptomycin. In 2021 there were eight of 30 fields with confirmed angular leaf spot, three fields containing resistant isolates. Correlations between environmental and/or management strategies and angular leaf spot incidence are currently being investigated via data analysis. (Reprinted with permission)