In order to develop a new natural fungal flavor, the exopolysaccharides (EPS) produced by the fermentation of Funalia trogii were prepared and characterized. The EPS was isolated and purified by Sepharose CL-6B and two fractions of the EPS were analyzed by FT-IR spectroscopy and applied into cigarette to determine their transfer ratio to cigarette smoke. The results showed that the absorption peaks appeared at 810, 880 and 910 cm^-1, it indicated the existence of mannose, and the co-existence of α and β glycoside bonds. The transfer ratio of EPS to cigarette smoke ranged from 1.58 % to 3.39 % with the application range of 0.02 % to 0.10 %.