Yellow sun-cured tobacco is a Chinese tobacco species that has been cultivated for several hundred years in China. Due to the special quality traits, yellow sun-cured tobacco is of great potential for developing Chinese style cigarettes with unique aroma and taste characteristics. On the surface or in the tissue of tobacco leaves, there are a large number of microbes, the metabolic activities of which may influence the transformation of chemical components throughout the curing process and thus the quality of tobacco leaves. The present study analysed the composition and dynamics of fungal communities of yellow sun-cured tobacco during curing by a combination of pure cultivation and molecular identification techniques. Cultivable fungi were preliminarily isolated from the early, middle and late stages of curing, using potato dextrose agar and sabouraud dextrose agar medium. The representative strains were then phylogenetically analyzed based on ITS sequences. A total of 126 fungi strains were isolated. The strains isolated from the middle leaves were relatively less compared with those from the bottom and upper leaves. In addition, the tobacco leaves seemed to contain more fungi in the early and late stages compared to those in the middle stage of curing. The 126 fungi strains were distributed in 2 phyla, 5 classes, 10 orders, 14 families and 17 genera. Ascomycota was the dominant phylum, with 93.65 % of the isolates belonging to this phylum, and Epicoccum, Arthrinium and Fusarium were the dominant genera, accounting for 23.02 %, 13.49 % and 11.90 % of the isolates, respectively. It was found that the fungal diversity of yellow sun-cured tobacco leaves during curing was relatively abundant, and the fungal composition varied in different leaf positions and dynamically changed as the curing process proceeded. The results of this study will shed light on improving tobacco quality traits by artificial regulation of the fungi community during the curing process.