CORESTA Congress, Paris, 2006, APOST 13

Genetic polymorphism of tobacco varieties based on ISSR markers

DEL PIANO L.; SORRENTINO C.; ABET M.; DI MURO A.; BARBATO L.; SICIGNANO M.; CUCINIELLO A.; COZZOLINO E.
Istituto Sperimentale per il Tabacco, Scafati, Italy
Tobacco is one of the most important non-food crops and numerous types having distinct morphological characters and chemical differences are grown commercially. At the present time, only limited information is available on the relationship between morphological variability and genetic diversity in different tobacco lines. In this paper Inter Simple Sequence Repeat (ISSR) molecular markers were used to detect the genetic polymorphism among N. tabacum varieties belonging to different tobacco types. Genomic DNA of thirty-six varieties was extracted and amplified utilizing 10 different 3' and 5' anchored primers previously selected for the reproducibility of the amplification profile. PCR products were separated on agarose gel, stained with ethidium bromide and visualized by UV transilluminator. The electrophoretic banding patterns were recorded and analyzed utilizing an image analysis system. The number of markers generated per primer ranged from 3 to 9, and the dimensions from 150 and 1700 bp. The amount of genetic polymorphism present among N. tabacum lines examined was limited as evidenced by the high degree of similarity in the ISSR profiles of the different tobaccos. These results until now obtained suggest that few genetic differences underlie the significant morphological variability observed among cultivated forms of tobacco. Further experiments of amplification with other primes are in progress.