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Tobacco Science & Technology, 2012, 3, p. 79-82, ISSN.1002-0861

Identification of Ralstonia solanacearum isolated from Burley planted soil and its biovars

WU Weiling(1); ZHANG Dinggui(2); YANG Shuiying(3); WANG Han(1); LI Hong(1); LI Zhenlun(1)
(1) College of Resource and Environment of Southwest University, Chongqing 400716, P.R. China; (2) Dazhou Branch of Sichuan Province Tobacco Company, Dazhou 635006, Sichuan, P.R. China; (3) College of Plant Protection of Southwest University, Chongqing 400716, P.R. China

In order to establish a method which is rapid and low in cost, for isolating and identifying the viable cells of Ralstonia solanacearum E.F. Smith (R. solanacearum) in burley planted soil, a semiselective medium (PCCG) combined with polymerase chain reaction (PCR) method were used. 12 strains were acquired, purified, and identified by internal transcribed spacer (ITS) region and BLAST in NCBI. The results showed that the 12 strains were highly homologous to R. solanacearum (92.6 %), which suggested that the method was suitable for the isolation and identification of R. solanacearum in soil. Furthermore, the results of Hayward’s classification criterion of R. solanacearum showed that the 12 strains all belonged to biovar Ⅲ.