With increasing interest in electronic cigarette testing, different e-cigarette aerosol collection approaches were investigated. The use of a machine designed specifically for e-cigarette aerosol generation and collection (Cerulean E-cigarette Testing Instrument 8, CETI8) was evaluated and compared to a standard smoking machine (Cerulean Linear Smoke Machine 450, SM450). Data will be presented that compares the aerosol generation and collection by both machines and highlights the novel features of the CETI8 for e-cigarette testing. Data for aerosol collected matter (ACM) and nicotine delivery will be presented using both aerosol collection systems. In addition, data illustrating the impact of collecting the e-cigarette aerosol in a conditioned environment versus a non-conditioned environment will be discussed. Lastly, results will be presented showing that the CETI8 is functionally equivalent to the SM450 for collection of e-cigarette aerosol, while providing additional e-cigarette specific capabilities for testing e-cigarettes.